Confirmation of GUS (uidA) and Cry1Ac Gene Transformation in Cotton (Gossypium hirusutum L.) Cultivars by GUS Histochemical Assay and PCR Analysis

Agrobacterium-Mediated Transformation of Pomegranate (Punica granatum L.) ‘Yousef Khani’ Using the gus Reporter Gene

In this study, an efficient Agrobacterium-mediated transformation method was developed forpomegranate (Punica granatum L.), a difficult-to-transform plant. In vitro shoot segments wereinoculated with Agrobacterium tumefaciens strain LBA4404 harboring the binary vectorpBI121 carrying the neomycin phosphotransferase (nptII) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....

Regeneration and Agrobacterium-mediated transformation of three economically important strawberry cultivars Kurdistan, Camarosa and Paros

Genetic transformation studies were carried out to standardize a protocol for Agrobacterium -mediated genetic transformation of three economically important strawberry (Fragaria x ananassa Duch) cultivars Kurdistan’, Camarosa’, and ‘Paros’. Shoot regeneration frequency 72, 65 and 30% was obtained on MS (1) basal medium supplemented with 2% glucose and 4 mg/l TDZ for Camarosa, Kurdistan and Paro...

PCR CONFIRMATION OF THE cry1Ac GENE IN TRANSGENIC BT (Bollgard) COTTON

Transgenic cotton (Gossypium hirsutum L.) with Bacillus thuringiensis Berliner δ-endotoxin genes has been widely adopted for suppression of lepidopteran pests. To account for inconsistency of the endotoxin gene expression, we developed a polymerase chain reaction (PCR) for checking the purity of transgenic cotton plants. A total of eight cry1Ac genes were aligned for the PCR primer design. A DN...

Gus Stadler

‘Liminal Dub’ by Gus Cummins

Images from ‘Dub in Liminal [4_TD$DIFF]Space’ produced in 2009–2010 towards MRes Digital Art and Technology at University of Plymouth. I embrace the ‘Dub [7_TD$DIFF]Reggae’ music genre. It strips the language from songs, sometimes returning it in a fragmented form, ornamented with delays and echoes. It provides an analogy for temporal lobe seizures; I experience dub versions of events, with lan...